Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Tapasin modification on the intracellular epitope HBcAg18-27 enhances HBV-specific CTL immune response and inhibits hepatitis B virus replication in vivo.

doi: 10.1038/labinvest.2014.6

Figure Lengend Snippet: Figure 3 Histopathologic changes and immunohistological analysis of hepatitis B surface antigen (HBsAg) and hepatitis B core antigen (HBcAg) in hepatitis B virus (HBV) transgenic mice livers. (a) Liver sections were stained with hematoxylin and eosin, and examined by light microscopy. Some lymphocytes appeared in the liver of mice. Representative photographs are presented (original magnifications: 400). (b and c) Liver sections were subjected to immunohistological analysis of HBsAg (b) and HBcAg (c). Representative photographs are presented (original magnifications: 400).

Article Snippet: For histological analysis, deparaffinized 3–5-mm-thick sections of the liver tissue were treated with 0.3% H2O2 in methanol for 10 min to eliminate the endogenous peroxidase activity, rinsed with distilled water, and immersed in PBS for 5 min. After the sections were blocked with normal goat serum for 30 min at room temperature, a goat anti-HBsAg polyclonal antibody and a goat anti-HBcAg polyclonal antibody (Novus Biologicals, USA) was applied overnight at 4 1C, and following three times of washing in PBS, sections were incubated for 30 min with biotinyated secondary antibody (Boster, China) at 37 1C and then for 30 min with streptavidin– biotin–peroxidase complex before being revealed by diaminobenzidine (DAB) and counterstained with hematoxylin.21 The Levels of Cytokine IFN-g and IL-2 Splenocytes (2 106 cells/ml) harvested from immunized HLA-A2 transgenic mice were cultured in 24-well plates at 37 1C in the presence of 10mg/ml HBcAg (CalBioreagents, USA).

Techniques: Virus, Transgenic Assay, Staining, Light Microscopy

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Tapasin modification on the intracellular epitope HBcAg18-27 enhances HBV-specific CTL immune response and inhibits hepatitis B virus replication in vivo.

doi: 10.1038/labinvest.2014.6

Figure Lengend Snippet: Figure 4 The levels of hepatitis B surface antigen (HBsAg), hepatitis B virus (HBV) DNA, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in HBV transgenic mice. The data are the means±s.d. of six mice per group. The HBsAg (a) and HBV DNA (b) inhibitory rate in sera of mice treated with 100 mg CTP-HBcAg18–27-Tapasin was significantly higher than those in 50 mg CTP-HBcAg18–27-Tapasin group. Data represent the means±s.d. (– ¼ 6) (*Po0.05). Meanwhile, the AST (c) and ALT (d) in sera of mice treated with 100 mg CTP-HBcAg18–27-Tapasin was significantly higher than those in 50 mg CTP-HBcAg18–27-Tapasin group. Data represent the means±s.d. (n ¼ 6) (*Po0.05). CTP, cytoplasmic transduction peptide.

Article Snippet: For histological analysis, deparaffinized 3–5-mm-thick sections of the liver tissue were treated with 0.3% H2O2 in methanol for 10 min to eliminate the endogenous peroxidase activity, rinsed with distilled water, and immersed in PBS for 5 min. After the sections were blocked with normal goat serum for 30 min at room temperature, a goat anti-HBsAg polyclonal antibody and a goat anti-HBcAg polyclonal antibody (Novus Biologicals, USA) was applied overnight at 4 1C, and following three times of washing in PBS, sections were incubated for 30 min with biotinyated secondary antibody (Boster, China) at 37 1C and then for 30 min with streptavidin– biotin–peroxidase complex before being revealed by diaminobenzidine (DAB) and counterstained with hematoxylin.21 The Levels of Cytokine IFN-g and IL-2 Splenocytes (2 106 cells/ml) harvested from immunized HLA-A2 transgenic mice were cultured in 24-well plates at 37 1C in the presence of 10mg/ml HBcAg (CalBioreagents, USA).

Techniques: Virus, Transgenic Assay, Transduction

Journal: Scientific Reports

Article Title: Hepatitis B virus genome replication triggers toll-like receptor 3-dependent interferon responses in the absence of hepatitis B surface antigen

doi: 10.1038/srep24865

Figure Lengend Snippet: Characteristics of HBV replication in HBVs-mut and HBV-s-rec strains.

Article Snippet: The following antibodies were used: anti-HBV core (HBcAg) (16), HBsAg antibody (Aviva Systems Biology, San Diego, CA, USA) and Gapdh (Cell Signaling, Danvers, MA, USA).

Techniques: